Pre-Clinical Publications
Establishing a New Platform to Investigate the Efficacy of Oncolytic Virotherapy in a Human Ex Vivo Peritoneal Carcinomatosis Model.
Viruses. 2023 Feb 27;15(2):363.
Jana Koch, Julia Beil, Susanne Berchtold, Dina Mönch, Annika Maaß, Irina Smirnow, Andrea Schenk, Mary E. Carter, Linus D. Kloker, Tobias Leibold, Philipp Renner, Marc-H Dahlke, Ulrich M. Lauer.
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A Three-Dimensional Organoid Model of Primary Breast Cancer to Investigate the Effects of Oncolytic Virotherapy
Front Mol Biosci. 2022; 9: 826302.
Mary E. Carter, Andreas D. Hartkopf, Anna Wagner, Léa L. Volmer, Sara Y. Brucker, Susanne Berchtold, Ulrich M. Lauer, André Koch
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Efficient Selection of Recombinant Fluorescent Vaccinia Virus Strains and Rapid Virus Titer Determination by Using a Multi-Well Plate Imaging System.
Biomedicines. 2021 Aug 18;9(8):1032.
Mingyu Ye, Markus Keicher, Ivaylo Gentschev, Aladar A. Szalay.
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Assessing and Overcoming Resistance Phenomena against a Genetically Modified Vaccinia Virus in Selected Cancer Cell Lines
Int J Mol Sci. 2020 Oct; 21(20):7618.
Susanne Berchtold, Julia Beil, Christian Raff, Irina Smirnow, Martina Schell, Janina D’Alvise, Silvia Gross, Ulrich M. Lauer
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Oncolytic vaccinia virus GLV-1h68 exhibits profound antitumoral activities in cell lines originating from neuroendocrine neoplasms
BMC Cancer. 2020; 20: 628.
Linus D. Kloker, Susanne Berchtold, Irina Smirnow, Julia Beil, Andreas Krieg, Bence Sipos, Ulrich M. Lauer
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Vaccinia virus injected human tumors: oncolytic virus efficiency predicted by antigen profiling analysis fitted boolean models
Bioengineered. 2019; 10(1): 190–196.
Alexander Cecil, Ivaylo Gentschev, Marion Adelfinger, Thomas Dandekar, Aladar A. Szalay
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PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma.
Clin Cancer Res. 2019 Jun 1;25(11):3443-3454.
Henry G. Smith, David Mansfield, Victoria Roulstone, Joan N. Kyula-Currie, Martin McLaughlin, Radhika R. Patel, Katharina F. Bergerhoff, James T. Paget, Magnus T. Dillon, Aadil Khan, Alan Melcher, Khin Thway, Kevin J. Harrington, Andrew J. Hayes.
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Chemovirotherapy of Pancreatic Adenocarcinoma by Combining Oncolytic Vaccinia Virus GLV-1h68 with nab-Paclitaxel Plus Gemcitabine
Mol Ther Oncolytics. 2017 Sep 15; 6: 10–21.
Eike Binz, Susanne Berchtold, Julia Beil, Martina Schell, Christine Geisler, Irina Smirnow, Ulrich M. Lauer
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Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis
Mol Ther Oncolytics. 2017 Jun 16; 5: 41–61.
Desislava Tsoneva, Boris Minev, Alexa Frentzen, Qian Zhang, Anja K. Wege, Aladar A. Szalay
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Colonization of xenograft tumors by oncolytic vaccinia virus (VACV) results in enhanced tumor killing due to the involvement of myeloid cells
J Transl Med. 2016; 14: 340.
Mehmet Okyay Kilinc, Klaas Ehrig, Maysam Pessian, Boris R. Minev, Aladar A. Szalay
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Oncolytic vaccinia virus combined with radiotherapy induces apoptotic cell death in sarcoma cells by down-regulating the inhibitors of apoptosis
Oncotarget. 2016 Dec 6; 7(49): 81208–81222.
Michelle J. Wilkinson, Henry G. Smith, Gráinne McEntee, Joan Kyula-Currie, Tim D. Pencavel, David C. Mansfield, Aadil A. Khan, Victoria Roulstone, Andrew J. Hayes, Kevin J. Harrington
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Isolated limb perfusion with biochemotherapy and oncolytic virotherapy combines with radiotherapy and surgery to overcome treatment resistance in an animal model of extremity soft tissue sarcoma
Int J Cancer. 2016 Sep 15; 139(6): 1414–1422.
Michelle J. Wilkinson, Henry G. Smith, Timothy D. Pencavel, David C. Mansfield, Joan Kyula‐Currie, Aadil A. Khan, Gráinne McEntee, Victoria Roulstone, Andrew J. Hayes, Kevin J. Harrington
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Silk-elastin-like protein polymer matrix for intraoperative delivery of an oncolytic vaccinia virus
PMC 2016 Aug 6. Published in final edited form as: Head Neck. 2016 Feb; 38(2): 237–246.
Daniel L. Price, Pingdong Li, Chun-Hao Chen, Danni Wong, Zhenkun Yu, Nanhai G. Chen, Yong A. Yu, Aladar A. Szalay, Joseph Cappello, Yuman Fong, Richard J. Wong
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Molecular network, pathway, and functional analysis of time-dependent gene changes associated with pancreatic cancer susceptibility to oncolytic vaccinia virotherapy
Mol Ther Oncolytics. 2016; 3: 16008.
Dana Haddad, Nicholas Socci, Chun-Hao Chen, Nanhai G Chen, Qian Zhang, Susanne G Carpenter, Arjun Mittra, Aladar A Szalay, Yuman Fong
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Oncolytic vaccinia virus as a vector for therapeutic sodium iodide symporter gene therapy in prostate cancer
Gene Ther. 2016 Apr; 23(4): 357–368.
D C Mansfield, J N Kyula, N Rosenfelder, J Chao-Chu, G Kramer-Marek, A A Khan, V Roulstone, M McLaughlin, A A Melcher, R G Vile, H S Pandha, V Khoo, K J Harrington
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Assessment of current virotherapeutic application schemes: “hit hard and early” versus “killing softly”?
Mol Ther Oncolytics. 2015; 2: 15018.
Benjamin Ruf, Ulrich M Lauer
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Drug-Encoded Biomarkers for Monitoring Biological Therapies
PLoS One.2015; 10(9): e0137573.
Desislava Tsoneva, Jochen Stritzker, Kristina Bedenk, Qian Zhang, Alexa Frentzen, Joseph Cappello, Utz Fischer, Aladar A. Szalay
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Oncolytic Gene Therapy with Recombinant Vaccinia Strain GLV-2b372 Efficiently Kills Hepatocellular Carcinoma
Surgery. PMC 2016 Aug 1. Published in final edited form as: Surgery. 2015 Aug; 158(2): 331–338.
Justin W Ady, Clark Johnsen, Kelly Mojica, Jacqueline Heffner, Damon Love, Amudhan Pugalenthi, Laurence J Belin, Nanhai G. Chen, Yong A. Yu, Aladar A. Szalay, Yuman Fong
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Preclinical Testing Oncolytic Vaccinia Virus Strain GLV-5b451 Expressing an Anti-VEGF Single-Chain Antibody for Canine Cancer Therapy
Viruses. 2015 Jul; 7(7): 4075–4092.
Marion Adelfinger, Simon Bessler, Alexa Frentzen, Alexander Cecil, Johanna Langbein-Laugwitz, Ivaylo Gentschev, Aladar A. Szalay
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Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus traps
J Transl Med. 2015; 13: 216.
Christina Kober, Susanne Rohn, Stephanie Weibel, Ulrike Geissinger, Nanhai G Chen, Aladar A Szalay
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Intratumoral INF-γ triggers an antiviral state in GL261 tumor cells: a major hurdle to overcome for oncolytic vaccinia virus therapy of cancer
Mol Ther Oncolytics. 2015; 2: 15009.
Christina Kober, Stephanie Weibel, Susanne Rohn, Lorenz Kirscher, Aladar A Szalay
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Doxycycline Inducible Melanogenic Vaccinia Virus as Theranostic Anti-Cancer Agent
Theranostics.2015; 5(10): 1045–1057.
Lorenz Kirscher, Xosé Luis Deán-Ben, Miriam Scadeng, Angelika Zaremba, Qian Zhang, Christina Kober, Thomas Felix Fehm, Daniel Razansky, Vasilis Ntziachristos, Jochen Stritzker, Aladar A. Szalay
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Expression of anti-VEGF antibody together with anti-EGFR or anti-FAP enhances tumor regression as a result of vaccinia virotherapy
Mol Ther Oncolytics. 2015; 2: 15003.
Ting Huang, Huiqiang Wang, Nanhai G Chen, Alexa Frentzen, Boris Minev, Aladar A Szalay
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Gene Therapy Using Therapeutic and Diagnostic Recombinant Oncolytic Vaccinia Virus GLV-1h153 for Management of Colorectal Peritoneal Carcinomatosis
Surgery. Author manuscript; available in PMC 2016 Feb 1. Published in final edited form as: Surgery. 2015 Feb; 157(2): 331–337.
Clarisse Eveno, Kelly Mojica, Justin W. Ady, Daniel L.J. Thorek, Valerie Longo, Laurence J. Belin, Sepideh Gholami, Clark Johnsen, Pat Zanzonico, Nanhai Chen, Tony Yu, Aladar A. Szalay, Yuman Fong
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Chemovirotherapy: combining chemotherapeutic treatment with oncolytic virotherapy
Oncolytic Virother. 2015; 4: 39–48.
Eike Binz, Ulrich M Lauer
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A Novel Vaccinia Virus with Dual Oncolytic and Anti-angiogenic Therapeutic Effects against Triple-Negative Breast Cancer
Breast Cancer Res Treat. Author manuscript; available in PMC 2017 Dec 11. Published in final edited form as: Breast Cancer Res Treat. 2014 Dec; 148(3): 489–499.
Sepideh Gholami, Andrew Marano, Nanhai G. Chen, Richard J. Aguilar, Alexa Frentzen, Chun-Hao Chen, Emil Lou, Sho Fujisawa, Clarisse Eveno, Laurence Belin, Pat Zanzonico, Aladar Szalay, Yuman Fong
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Correction in: Breast Cancer Res Treat. 2016 Apr; 156(3): 607–608.
Combination immunotherapy with oncolytic vaccinia virus and checkpoint inhibitor following local tumor irradiation
J Immunother Cancer.2014; 2(Suppl 3): P112.
Boris Minev, Holbrook Kohrt, Mehmet Kilinc, Nanhai Chen, Audrey Feng, Maysam Pessian, Ulrike Geissinger, Erik Haefner, Desislava Tsoneva, Kliment Bozhilov, Idit Sagiv-Barfi, Xing Zhao, Narendiran Rajesekaran, Ronald Levy, Aladar Szalay
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Inducible Gene Expression in Tumors Colonized by Modified Oncolytic Vaccinia Virus Strains
J Virol.2014 Oct; 88(19): 11556–11567.
Jochen Stritzker, Sascha Huppertz, Qian Zhang, Ulrike Geissinger, Barbara Härtl, Ivaylo Gentschev, Aladar A. Szalay
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Evaluation of a New Recombinant Oncolytic Vaccinia Virus Strain GLV-5b451 for Feline Mammary Carcinoma Therapy
PLoS One.2014; 9(8): e104337.
Marion Adelfinger, Ivaylo Gentschev, Julio Grimm de Guibert, Stephanie Weibel, Johanna Langbein-Laugwitz, Barbara Härtl, Hugo Murua Escobar, Ingo Nolte, Nanhai G. Chen, Richard J. Aguilar, Yong A. Yu, Qian Zhang, Alexa Frentzen, Aladar A. Szalay
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Antigen profiling analysis of vaccinia virus injected canine tumors: Oncolytic virus efficiency predicted by boolean models
Bioengineered. 2014 Sep 1; 5(5): 319–325.
Alexander Cecil, Ivaylo Gentschev, Marion Adelfinger, Ingo Nolte, Thomas Dandekar, Aladar A Szalay
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Oncolytic Immunotherapy Using Recombinant Vaccinia Virus GLV-1h68 Efficiently Kills Sorafenib-Resistant Hepatocellular Carcinoma
Surgery. 2014 Aug; 156(2): 263–269.
Justin W. Ady, Jacqueline Heffner, Kelly Mojica, Clark Johnsen, Laurence J. Belin, Damon Love, Amudhan Pugalenthi, Elizabeth Klein, Nanhai G. Chen, Yong A. Yu, Aladar A. Szalay, Yuman Fong
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Combination treatment with oncolytic Vaccinia virus and cyclophosphamide results in synergistic antitumor effects in human lung adenocarcinoma bearing mice
J Transl Med. 2014 July 17; 12-197.
Elisabeth Hofmann, Stephanie Weibel, Aladar A Szalay
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Characterization of Metastasis Formation and Virotherapy in the Human C33A Cervical Cancer Model
PLoS One.2014; 9(6): e98533. Published online 2014 Jun 2. doi: 10.1371/journal.pone.0098533
Ulrike Donat, Juliane Rother, Simon Schäfer, Michael Hess, Barbara Härtl, Christina Kober, Johanna Langbein-Laugwitz, Jochen Stritzker, Nanhai G. Chen, Richard J. Aguilar, Stephanie Weibel, Aladar A. Szalay
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Oncolytic Virotherapy of Canine and Feline Cancer
Viruses.2014 May; 6(5): 2122–2137. Published online 2014 May 16. doi: 10.3390/v6052122
Ivaylo Gentschev, Sandeep S. Patil, Ivan Petrov, Joseph Cappello, Marion Adelfinger, Aladar A. Szalay
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PEG-pHPMAm-based polymeric micelles loaded with doxorubicin-prodrugs in combination antitumor therapy with oncolytic vaccinia viruses
Polym Chem. 2014 Mar 7; (5): 1674–1681.
Eduardo Ruiz-Hernández, Michael Hess, Gustavo J. Melen, Benjamin Theek, Marina Talelli, Yang Shi, Burcin Ozbakir, Erik A. Teunissen, Manuel Ramírez, Diana Moeckel, Fabian Kiessling, Gert Storm, Hans W. Scheeren, Wim E. Hennink, Aladar A. Szalay, Jochen Stritzker, Twan Lammers
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Vaccinia virus GLV-1h153 in combination with 131I shows increased efficiency in treating triple-negative breast cancer
FASEB J. 2014 Feb; 28(2): 676–682. doi: 10.1096/fj.13-237222
Sepideh Gholami, Chun-Hao Chen, Emil Lou, Laurence J. Belin, Sho Fujisawa, Valerie A. Longo, Nanhai G. Chen, Mithat Gönen, Pat B. Zanzonico, Aladar A. Szalay, Yuman Fong
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A novel oncolytic viral therapy and imaging technique for gastric cancer using a genetically engineered vaccinia virus carrying the human sodium iodide symporter
Journal of Experimental & Clinical Cancer Research 2014, 33:2.
Kyong-Hwa Jun, Sepideh Gholami, Tae-Jin Song, Joyce Au, Dana Haddad, Joshua Carson, Chun-Hao Chen, Kelly Mojica, Pat Zanzonico, Nanhai G Chen, Qian Zhang, Aladar Szalay and Yuman Fong.
Targeting hematologic malignancies with oncolytic vaccinia virus constructs
J Immunother Cancer.2013; 1(Suppl 1): P226. Published online 2013 Nov 7. doi: 10.1186/2051-1426-1-S1-P226
Nanhai Chen, Mehmet Kilinc, Qian Zhang, Jason Aguilar, Desislava Tsoneva, Maysam Pessian, Boris Minev, Aladar Szalay
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Optical Detection and Virotherapy of Live Metastatic Tumor Cells in Body Fluids with Vaccinia Strains
PLoS One. 2013; 8(9): e71105. Published online 2013 Sep 3. doi: 10.1371/journal.pone.0071105
Huiqiang Wang, Nanhai G. Chen, Boris R. Minev, Martina Zimmermann, Richard J. Aguilar, Qian Zhang, Julia B. Sturm, Falko Fend, Yong A. Yu, Joseph Cappello, Ulrich M. Lauer, Aladar A. Szalay
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An Oncolytic Vaccinia Virus Expressing the Human Sodium Iodine Symporter Prolongs Survival and Facilitates SPECT/CT Imaging in an Orthotopic Model of Malignant Pleural Mesothelioma
Surgery. Author manuscript; available in PMC 2014 Sep 1.
Published in final edited form as: Surgery. 2013 Sep; 154(3): 486–495. Published online 2013 Jul 23. doi: 10.1016/j.surg.2013.06.004
Laurence J. Belin, Justin W. Ady, Christina Lewis, Drew Marano, Sepideh Gholami, Kelly Mojica, Clarisse Eveno, Valerie Longo, Pat B. Zanzonico, Nanhai G. Chen, Aladar A. Szalay, Yuman Fong
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Vaccinia Virus-mediated Expression of Human Erythropoietin in Tumors Enhances Virotherapy and Alleviates Cancer-related Anemia in Mice
Mol Ther.2013 Nov; 21(11): 2054–2062. Prepublished online 2013 Jun 14. Published online 2013 Jul 2. doi: 10.1038/mt.2013.149
Duong H Nguyen, Nanhai G Chen, Qian Zhang, Ha T Le, Richard J Aguilar, Yong A Yu, Joseph Cappello, Aladar A Szalay
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Radiation lays down covering fire for viral shock troops
Oncotarget. 2013 Sep; 4(9): 1329–1330. Published online 2013 Jul 19. doi: 10.18632/oncotarget.1187
Joan N. Kyula, Aadil A. Khan, Michelle J. Wilkinson, David Mansfield, Kevin J. Harrington
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Vaccinia virus expressing bone morphogenetic protein-4 in novel glioblastoma orthotopic models facilitates enhanced tumor regression and long-term survival
J Transl Med. 2013; 11: 155. Published online 2013 Jun 24. doi: 10.1186/1479-5876-11-155
Rohit Duggal, Ulrike Geissinger, Qian Zhang, Jason Aguilar, Nanhai G Chen, Elena Binda, Angelo L Vescovi, Aladar A Szalay
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Single-agent combinatorial cancer therapy
Proc Natl Acad Sci U S A. 2013 May 21; 110(21): 8325–8326. Published online 2013 May 10. doi: 10.1073/pnas.1305832110
Jochen Stritzker, Aladar A. Szalay
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Treatment of malignant effusion by oncolytic virotherapy in an experimental subcutaneous xenograft model of lung cancer
J Transl Med. 2013; 11: 106. Published online 2013 May 1. doi: 10.1186/1479-5876-11-106
Stephanie Weibel, Elisabeth Hofmann, Thomas Christian Basse-Luesebrink, Ulrike Donat, Carolin Seubert, Marion Adelfinger, Prisca Gnamlin, Christina Kober, Alexa Frentzen, Ivaylo Gentschev, Peter Michael Jakob, Aladar A Szalay
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Growth inhibition of different human colorectal cancer xenografts after a single intravenous injection of oncolytic vaccinia virus GLV-1h68
J Transl Med.2013; 11: 79. Published online 2013 Mar 26. doi: 10.1186/1479-5876-11-79
Klaas Ehrig, Mehmet O Kilinc, Nanhai G Chen, Jochen Stritzker, Lisa Buckel, Qian Zhang, Aladar A Szalay
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Growth inhibition of different human colorectal cancer xenografts after a single intravenous injection of oncolytic vaccinia virus GLV-1 h68
J Transl Med. 2013 Mar 26;11(1):79.
Ehrig K, Kilinc MO, Chen NG, Stritzker J, Buckel L, Zhang Q, Szalay AA.
Vaccinia Virus GLV1h-153 is a Novel Agent for Detection and Treatment/Local Control of Positive Surgical Margins for Breast Cancer
Breast Cancer Res. 2013 Mar 18;15(2):R26.
Gholami S, Chen CH, Belin L, Lou E, Antonacci C, Carew A, Chen NG, De Brot M, Longo V, Zanzonico PB, Szalay AA, Fong Y.
Vaccinia virus-mediated melanin production allows MR and optoacoustic deep tissue imaging and laser-induced thermotherapy of cancer
Proc Natl Acad Sci U S A.2013 Feb 26; 110(9): 3316–3320. Published online 2013 Feb 11. doi: 10.1073/pnas.1216916110
Jochen Stritzker, Lorenz Kirscher, Miriam Scadeng, Nikolaos C. Deliolanis, Stefan Morscher, Panagiotis Symvoulidis, Karin Schaefer, Qian Zhang, Lisa Buckel, Michael Hess, Ulrike Donat, William G. Bradley, Vasilis Ntziachristos, Aladar A. Szalay
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Oncolytic vaccinia therapy of salivary gland carcinoma.
JAMA Otolaryngol Head Neck Surg. 2013 Feb 1;139(2):173-82.
Chernichenko N, Linkov G, Li P, Bakst RL, Chen CH, He S, Yu YA, Chen NG, Szalay AA, Fong Y, Wong RJ.
Imaging of intratumoral inflammation during oncolytic virotherapy of tumors by 19F-magnetic resonance imaging (MRI)
PLoS One. 2013;8(2):e56317.
Weibel S, Basse-Luesebrink TC, Hess M, Hofmann E, Seubert C, Langbein-Laugwitz J, Gentshev I, Sturm VJF, Ye Y, Kampf T, Jakob PM, Szalay AA.
A vaccinia virus encoding the human sodium iodide symporter facilitates long-term image monitoring of virotherapy and targeted radiotherapy of pancreatic cancer.2012.
J Nucl Med. 2012 53:1933-1942
Haddad D, Zanzonic, PB, Carlin S, Chen C H, Chen NG, Zhang Q, Yu Y A, Longo V, Mojica K, Aguilar R J, Szalay AA, Fong Y.
Characterization and evaluation of a new oncolytic Vaccinia Virus strain LIVP6.1.1 for canine cancer therapy.2013.
Bioengineered. 2013;4(2):84-
Gentschev I, Patil S, Adelfinger M, Weibel S, Geissinger U, Frentzen A, Chen NG, Yu YA, Zhang Q, Ogilvie G, Szalay AA.
Virotherapy of Canine Tumors with Oncolytic Vaccinia Virus GLV-1h109 Expressing an Anti-VEGF Single-Chain Antibody. 2012.
PLoS One. 2012;7(10):e47472.
Patil SS, Gentschev I, Adelfinger M, Donat U, Hess M, Weibel S, Nolte I, Frentzen A, Szalay AA.
Correlates between host and viral transcriptional program associated with different oncolytic vaccinia virus isolates. 2012
Hum Gene Ther Methods. 2012 Oct 17.
Reinboth J, Ascierto ML, Chen NG, Zhang Q, Yu YA, Aguilar RJ, Carretero R, Worschech A, Zhao Y, Wang E, Marincola FM, Szalay AA.
Preferential Colonization of Metastases by Oncolytic Vaccinia Virus Strain GLV-1h68 in a Human PC-3 Prostate Cancer Model in Nude Mice. 2012.
PLoS One 7(9):e45942.
Donat U, Weibel S, Hess M, Stritzker J, Härtl B, Sturm JB, Chen NG, Gentschev I, Szalay AA
Vaccinia virus-mediated intra-tumoral expression of matrix metalloproteinase 9 enhances oncolysis of PC-3 xenograft tumors. 2012
BMC Cancer. 2012 Aug 23;12(1):366.
Schaefer S, Weibel S, Donat U, Zhang Q, Aguilar RJ, Chen NG, Szalay AA.
Imaging characteristics, tissue distribution, and spread of a novel oncolytic vaccinia virus carrying the human sodium iodide symporter. 2012.
PLoS One. 2012;7(8):e41647. Epub 2012 Aug 17.
Haddad D, Chen CH, Carlin S, Silberhumer G, Chen NG, Zhang Q, Longo V, Carpenter SG, Mittra A, Carson J, Au J, Gonen M, Zanzonico PB, Szalay AA, Fong Y.
Oncolytic vaccinia virus GLV-1h68 strain shows enhanced replication in human breast cancer stem-like cells in comparison to breast cancer cells. 2012.
J Transl Med. 2012 Aug 17;10(1):167.
Wang H, Chen NG, Minev BR, Szalay AA.
Vaccinia Virus GLV-1h153 is Effective in Treating and Preventing Metastatic Triple-Negative Breast Cancer 2012
Ann Surg. 2012 Sep;256(3):437-445.
Gholami S, Chen CH, Lou E, De Brot M, Fujisawa S, Chen NG, Szalay AA, Fong Y
Published
Preclinical evaluation of oncolytic vaccinia virus for therapy of canine soft tissue sarcoma. 2012
PLoS ONE 2012;7(5):e37239.
Gentschev I, Adelfinger M, Josupeit R, Rudolph S, Ehrig K, Weibel S, Chen NG, Yu YA, Zhang Q, Thamm D, Stritzker J, MacNeill A, Szalay AA.
Preferential replication of systemically delivered oncolytic vaccinia virus in focally irradiated glioma xenografts. 2012
Clin Cancer Res. 2012 May 1;18(9):2579-90.
Advani SJ, Buckel L, Chen NG, Scanderbeg DJ, Geissinger U, Zhang Q, Yu YA, Aguilar
Effective oncolytic vaccinia therapy for human sarcomas. 2012
J Surg Res. 2012 Jun 15;175(2):e53-60.
He S, Li P, Chen CH, Bakst RL, Chernichenko N, Yu YA, Chen N, Szalay AA, Yu Z, Fong Y, Wong RJ.
A novel genetically modified oncolytic vaccinia virus in experimental models is effective against a wide range of human cancers. 2012
Ann Surg Oncol. 2012;19:665-674.
Haddad D, Chen N, Zhang Q, Chen CH, Yu YA, Gonzalez L, Aguilar J, Li P, Wong J, Szalay AA, Fong Y.
BACKGROUND: Replication-competent oncolytic viruses have shown great promise as a potential cancer treatment. This study aimed to determine whether a novel vaccinia virus, GLV-1h151, with genetic modifications enhancing cancer specificity and enabling virus detection, is effective against a range of human cancers and is safe when administered in preclinical models.
Functional hyper-IL-6 from vaccinia virus-colonized tumors triggers platelet formation and helps to alleviate toxicity of mitomycin C enhanced virus therapy. 2012
J Transl Med. 2012 Jan 11;10(1):9.
Sturm JB, Hess M, Weibel S, Chen NG, Yu YA, Zhang Q, Donat U, Reiss C, Gambaryan S, Stritzker J, A. A. Szalay AA.
Oncolytic virotherapy in veterinary medicine: current status and future prospects for canine patients. 2012.
Journal of Translational Medicine. 10:3
Sandeep S. Patil, Ivaylo Gentsche v, Ingo Nolte, Gregory Ogilvie, and Aladar A. Szalay
Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68. 2011.
BMC Cancer. Oct 19;11:451.
Ascierto ML, Worschech A, Yu Z, Adams S, Reinboth J, Chen NG, Pos Z, Roychoudhuri R, Di Pasquale G, Bedognetti D, Uccellini L, Rossano F, Ascierto PA, Stroncek DF, Restifo NP, Wang E, Szalay AA, Marincola FM.
Novel therapy for anaplastic thyroid carcinoma cells utilizing an oncolytic vaccinia virus carrying the human sodium iodide symporter. 2011.
Surgery. 150: 1040-7
Sepideh Gholami, Dana Haddad, Chun-Hao Chen, Nanhai G. Chen, Qian Zhang, Pat B. Zanzonico, and Aladar A. Szalay, Yuman Fong
Bacterial glucuronidase as general marker for oncolytic virotherapy or other biological therapies. 2011.
Journal of Translational Medicine. 9:172.
Michael Hess, Jochen Stritzker, Barbara Härtl, Julia B. Sturm, Ivaylo Gentschev and Aladar A. Szalay
Magnetic Resonance Imaging of tumors colonized with bacterial ferritin-expressing Escherichia coli. 2011.
PLoS ONE. Vol. 6:10.
Philip Hill, Jochen Stritzker, Miriam Scadeng, Ulrike Geissinger, Daniel Haddad, Thomas Basse-Lusebrink, Uwe Gbureck, Peter Jakob, Aladar Szalay.
Replication efficiency of oncolytic vaccinia virus in cell cultures prognosticates the virulence and antitumor efficacy in mice. 2011.
Journal of Translational Medicine. 9:164.
Nanhai G. Chen, Yong A. Yu, Qian Zhang and Aladar A. Szalay
Efficient colonization and therapy of human hepatocellular carcinoma (HCC) using the oncolytic vaccinia virus strain GLV-1h68. 2011.
PLoS ONE. Vol. 6:7, e22069.
Ivaylo Gentschev, Meike Müller, Marion Adelfinger, Stephanie Weibel, Friedrich Grummt, Martina Zimmermann, Michael Bitzer, Qian Zhang, Yong A. Yu, Nanhai G. Chen, Jochen Stritzker, Ulrich M. Lauer, Aladar A. Szalay
Insertion of the human sodium iodide symporter to facilitate deep tissue imaging does not alter oncolytic or replication capability of a novel vaccinia virus. 2011.
Journal of Translational Medicine. 9:36.
Dana Haddad, Nanhai G Chen, Qian Zhang, Chun-Hao Chen, Yong A Yu, Lorena Gonzalez, Susanne G Carpenter, Joshua Carson, Joyce Au, Arjun Mittra, Mithat Gonen, Pat B Zanzonico, Yuman Fong and Aladar A Szalay
Vaccinia Virus GLV-1h237 carrying a Walker A motif mutation of mouse Cdc6 protein enhances human breast tumor therapy in mouse xenografts. 2011.
International Journal of Oncology. 38(3):871-8.
Hofmann E, Grummt F, Szalay A.A.
Viral-mediated oncolysis is the most critical factor in the late-phase of the tumor regression process upon vaccinia virus infection. 2011.
BMC Cancer. 11:68
Stephanie Weibel, Viktoria Raab, Yong Yu, Andrea Worschech, Ena Wang, Francesco Marincola, and Aladar Szalay
Oncolytic Virotherapy of Cancer. 2011.
Cancer Management in Man: Chemotherapy, Biological Therapy, Hyperthermia and Supporting Measures, Cancer Growth and Progression. 295-316.
Chen N.G., Szalay A.A.
Oncolytic vaccinia virus: a theranostic agent for cancer. 2010.
Future Virology. November 2010, Vol. 5, No. 6, Pages 763-784.
Chen N.G., Szalay A. A.
Enhanced tumor therapy using vaccinia virus strain GLV-1h68 in combination with a β-galactosidase-activatable prodrug seco-analog of duocarmycin SA. (2011)
Cancer Gene Therapy. 2011;18:42-52.
Seubert CM, Stritzker J, Hess M, Donat U, Sturm JB, Chen N, Hof JM, Krewer B, Tietze LF, Gentschev I, Szalay AA.
Enterobacterial tumor colonization in mice depends on bacterial metabolism and macrophages but is independent of chemotaxis and motility. (2010)
Int J Med Microbiol. 2010;300(7):449-56.
Stritzker J, Weibel S, Seubert C, Götz A, Tresch A, van Rooijen N, Oelschlaeger TA, Hill PJ, Gentschev I, Szalay AA.
Significant growth inhibition of canine mammary carcinoma xenografts following treatment with oncolytic vaccinia virus GLV-1h68. (2010)
Journal of Oncology. 2010:736907, 10.
Gentschev I, Ehrig K, Donat U, Hess M, Rudolph S, Chen N, Yu Y.A., Zhang Q, Bullerdiek J, Nolte I, Stritzker J, Szalay AA.
Regression of human prostate tumors and mestastases in nude mice following treatment with the recombinant oncolytic vaccinia virus GLV-1h68. (2010)
Journal of Biomedicine and Biotechnology. 2010: 489759.
Gentschev I, Donat U, Hofmann E, Weibel S, Adelfinger M, Raab V, Heisig M, Chen N, Yu YA, Stritzker J, Szalay AA.
Myristoylation negative msbB-mutants of probiotic E. coli Nissle 1917 retain tumor specific colonization properties but show less side effects in immunocompetent mice. (2010)
Bioengineered Bugs. 1:2, 139-145:March/April 2010.
Stritzker J, Hill P, Gentschev I, Szalay AA.
The highly attenuated oncolytic recombinant vaccinia virus GLV-1h68: Comparative genomic features and the contribution of F14.5L inactivation (2009)
Mol Genet Genomics. 2009 Oct;282(4):417-35
Zhang Q, Liang C, Yu YA, Chen N, Dandekar T, Szalay AA.
Oncolytic vaccinia viral therapy of squamous cell carcinoma (2009)
Molecular Cancer.
Yu Z, Li S, Brader P, Chen N, Yu YA, Zhang Q, Szalay AA, Fong Y, Wong RJ.
Anti-VEGF single chain antibody GLAF-1 encoded by oncolytic vaccinia virus significantly enhances therapy in colonized tumor xenografts (2009)
PNAS.
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Imaging a Genetically Engineered Oncolytic Vaccinia Virus (GLV-1h99) Using a Human Norepinephrine transporter (hNET) Report Gene (2009)
Clinical Cancer Research. 15(11):3791-3801.
Brader P, Kelly K, Chen N, Yu YA, Zhang Q, Zanzonico P, Burnazi EM, Ghani ER, Serganova I, Hricak H, Szalay AA, Fong Y, Blasberg R.
Systemic treatment of xenografts with vaccinia virus GLV-1h68 reveals the immunologic facet of oncolytic therapy (2009)
BMC Genomics.
Andrea Worschech, Nanhai Chen, Yong A Yu, Qian Zhang, Zoltan Pos, Stephanie Weibel, Viktoria Raab, Marianna Sabatino, Alessandro Monaco, Hui Liu, Vladia Monsurró, R. Mark Buller, David F Stroncek, Ena Wang, Aladar A Szalay, Francesco M Marincola.
The immunologic aspects of poxvirus oncolytic therapy. (2009)
Cancer Immunology, Immunotherapy. 58:1355-1362.
A. Worschech, D. Haddad, DF. Stroncek, E. Wang, F. M. Marincola, A. A. Szalay.
A novel recombinant vaccinia virus expressing the human norepinephrine transporter retains oncolytic potential and facilitates deep tissue imaging. (2009)
Molecular Medicine. 15 (5-6) 144-151.
Nanhai Chen, Qian Zhang, Yong A. Yu, Peter Brader, Inna Serganova, Ronald Blasberg, Yuman Fong, and Aladar A. Szalay.
Real-time intraoperative detection of melanoma lymph node metastases using recombinant vaccinia virus GLV-1h68 in an immunocompetent animal model. (2009)
International Journal of Cancer. 124:911-918.
Kelly K, Woo Y, Brader P, Li S, Chen N, Yu YA, Szalay AA, Fong Y.
Regression of human pancreatic tumor xenografts in mice after a single systemic injection of recombinant vaccinia virus GLV-1h68. (2009)
Molecular Cancer Therapeutics. 8:141-151.
Yu YA, Galanis C, Woo Y, Chen N, Zhang Q, Fong Y, Szalay AA.
Use of an oncolytic vaccinia virus for treatment of canine breast cancer in nude mice: preclinical development of a therapeutic agent. (2008)
Cancer Gene Therapy. 16(4):320-8.
Gentschev I, Stritzker J, Hofmann E, Weibel S, Yu YA, Chen N, Zhang Q, Bullerdiek J, Nolte I, Szalay AA.
Oncolytic Vaccinia Therapy for Anaplastic Thyroid Cancer In Vivo. (2008)
Journal of Clinical Endocrinology and Metabolism. 93(11):4403-4407.
Lin SF, Price DL, Chen CH, Brader P, Li S, Gonzalez L, Zhang Q, Yu YA, Chen N, Szalay AA, Fong Y, Wong RJ.
Novel oncolytic agent, GLV-1h68, is effective against malignant pleural mesothelioma. (2008)
Human Gene Therapy. 19:774-782.
Kelly K, Woo Y, Brader P, Yu Z, Riedl C, Lin S, Chen N, Yu YA, Rusch VW, Szalay AA, Fong Y.
Colonization of experimental murine breast tumors by E. coli K-12 significantly alters the tumor microenvironment. (2008)
Cellular Microbiology. 10(6), 1235-1248.
Weibel S, Stritzker J, Eck M, Goebel W, Szalay AA.
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Establishment and characterization of conditions required for tumor colonization by intravenously delivered bacteria. (2008)
Biotechnology and Bioengineering. 100(3), 567-578.
Yu YA, Zhang Q, Szalay AA.
Prodrug converting enzyme gene delivery by L. monocytogenes. (2008)
BMC Cancer. 8:94.
Stritzker J, Pilgrim S, Szalay AA, Goebel W.
Escherichia coli Nissle 1917 facilitates tumor detection by positron emission tomography and optical imaging. (2008)
Clinical Cancer Research. 14(8), 2295-2302.
Brader P, Stritzker J, Riedl CC, Zanzonico P, Cai S, Burnazi EM, Ghani ER, Hricak H, Szalay AA, Fong Y, Blasberg R.
Signatures Associated with Rejection or Recurrence in HER-2/neu-Positive Mammary Tumors.
Cancer Research. 68(7), 2436-46.
Worschech A, Kmieciak M, Keeler JM, Bear HD, Szalay AA, Wang E, Marincola FM, Manjili MH
Treatment of anaplastic thyroid carcinoma in vitro with a mutant vaccinia virus. (2007)
Surgery. 14 (6): 976-983.
Shu-Fu L, Yu Z, Riedl C, Woo Y, Zhang Q, Yu YA, Timiryasova T, Chen N, Shah JP, Szalay AA, Fong Y, Wong RJ
E. coli Nissle 1917: Vom Kriegsveteran weiterentwickelt zum aktiven Kämpfer gegen Tumoren? [E. coli Nissle 1917: From War Veteran to Genetically-Directed Tumor Fighter.] (2007)
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Eradication of Solid Human Breast Tumors in Nude Mice with an Intravenously Injected Light-Emitting Oncolytic Vaccinia Virus. (2007)
Cancer Research. 67 (20), 10038-46.
Zhang Q, Yu YA, Wang E, Chen N, Danner RL, Munson PJ, Marincola FM, Szalay AA.
Proceedings of the 14th International Symposium on Bioluminescence & Chemiluminescence: Chemistry, Biology and Applications. (2007)
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Real-time monitoring of vaccinia virus infection in cultured cells and in living mice using light-emitting proteins. (2007)
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Chen N, Yu YA, Zhang Q, Raab V, Szalay AA.
Four-color labeling of cell culture and tumors of live mice upon infection with: GFP-Ruc and RFP-CBG99 expressing Vaccinia virus strains. (2007)
In: Proceedings of the 14th International Symposium on Bioluminescence & Chemiluminescence: Chemistry, Biology and Applications.
World Scientific: Singapore, 2007: 197-200.
Raab V, Horbaschek C, Chen N, Zhang Q, Yu YA, Seubert C, Geissinger U, Worschech A, Tietze CJ, Zellner E, Le TH, Grummt F, Stritzker J, Szalay AA.
Evaluation of an in vivo gene induction system in infected tumor-bearing mice. (2007)
In: Proceedings of the 14th International Symposium on Bioluminescence & Chemiluminescence: Chemistry, Biology and Applications.
World Scientific: Singapore, 2007: 205-208.
Stritzker J, Weibel S, Hill PJ, Szalay AA.
Examinations of bacterium-mediated detection of tumors in mice models. (2007)
In: Proceedings of the 14th International Symposium on Bioluminescence & Chemiluminescence: Chemistry, Biology and Applications.
World Scientific: Singapore, 2007: 209-212.
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Tumor-specific colonization, tissue distribution, and gene induction by probiotic E. coli Nissle 1917 in live mice. (2007)
International Journal of Medical Microbiology. 297(3):151-62.
Stritzker J, Weibel S, Hill PJ, Oelschlaeger TA, Goebel W, Szalay AA.
Visualization of tumors and metastases in live animals with bacteria and vaccinia virus encoding light-emitting proteins. (2004)
Nature Biotechnology. 22, 313-320.
Yu YA, Shabahang S, Timiryasova T, Zhang Q, Beltz R, Gentschev I, Goebel W, Szalay AA.
Optical imaging: bacteria, viruses, and mammalian cells encoding light-emitting proteins reveal the locations of primary tumors and metastases in animals. (2003)
Analytical and Bioanalytical Chemistry. 377, 964-972.
Yu YA, Timiryasova T, Zhang Q, Beltz R, Szalay AA.